目的 探究丹参多酚酸对脑梗死大鼠神经血管微循环及缺血侧血流的影响。方法 将大鼠随机分为假手术组、模型组、尼莫地平10 mg/kg组和丹参多酚酸低、高剂量组（10、25 mg/kg）。制备大鼠中动脉缺血模型，通过神经功能评分、2，3，5-三苯基氯化四氮唑染色以及采用激光散斑血流成像检测脑血流量，鉴定模型构建是否成功。各组大鼠按照分组进行给药，尼莫地平组ig尼莫地平10 mg/kg，丹参多酚酸组ip 10、25 mg/kg注射用丹参多酚酸，连续治疗7 d，1次/d。取脑组织分别进行2,3,5-三苯基氯化四氮唑染色染色评估脑梗死体积，伊文思蓝含量测定评估血、脑、脊液屏障结构完整性，免疫荧光染色测量血小板内皮细胞黏附分子（CD31）表达。采用Western blotting实验检测血管内皮生长因子（VEGF）、血管生成素-1（Ang-1）、磷酸甘油醛脱氢酶（GAPDH）蛋白表达。结果 与模型组相比，丹参多酚酸25 mg/kg组神经功能评分、脑梗死体积占比明显减低，脑血流量明显增加（P＜0.05）；且缺血侧CD31相对表达明显减少；伊文思蓝含量以及VEGF与Ang1蛋白表达明显增加（P＜0.05）。结论 丹参多酚酸能够明显促进神经血管微循环重构，加速缺血区血流恢复，最终减轻缺血性卒中损伤作用，其机制可能与VEGF与Ang1蛋白表达增加相关。

Objective To investigate the effects of salvianolic acids on neural angiogenesis and cerebral blood flow in rats with ischemic stroke. Methods Rats were randomly divided into sham operation group, model group, nimodipine 10 mg/kg group, and salvianolic acid low and high-dose groups (10 and 25 mg/kg). The rat model of middle artery ischemia was prepared, and the cerebral blood flow was detected by nerve function score, 2,3,5-triphenyltetrazolium chloride staining and laser speckle flow imaging to determine whether the model was successfully constructed. The rats in each group were given medication according to the groups, nimodipine group ig Nimodipine Tablets 10 mg/kg, salvianolic acid group ip 10, 25 mg/kg Salvianolic acid for injection, continuous treatment for 7 d, once daily. 2,3,5-triphenyltetrazazole chloride staining was performed on the brain tissues to evaluate the infarct volume, evans blue content was determined to evaluate the structural integrity of the blood, brain, and spinal fluid barrier, and the expression of platelet endothelial cell adhesion molecule (CD31) was measured by immunofluorescence staining. The protein expressions of vascular endothelial growth factor (VEGF), Ang-1, and phosphoglyceraldehyde dehydrogenase (GAPDH) were detected by Western blotting assay. Results Compared with model group, 25 mg/kg salvianolic acid group significantly decreased the neurological function score and cerebral infarction volume ratio, and significantly increased cerebral blood flow (P < 0.05). The relative expression of CD31 in ischemic side was significantly decreased, Evans blue content and the expression of VEGF and Ang1 protein were significantly increased (P < 0.05). Conclusion Salvianolic acids can significantly promote neurovascular microcirculation remodeling, accelerate the recovery of blood flow in the ischemic area, and ultimately reduce the injury of ischemic stroke. The mechanism may be related to the increase of VEGF and Ang1 protein expression.

R971

海南省卫生健康行业科研项目（22A200042）