目的 建立测定芪桂痛风片中新绿原酸、秦皮甲素、绿原酸、秦皮乙素、秦皮苷、秦皮素、4,5-O-二咖啡酰奎宁酸、蒙花苷的HPLC方法。方法 采用ACE Excel C₁₈-AR色谱柱（150 mm×4.6 mm，3 μm）；流动相为0.1%甲酸水–乙腈，梯度洗脱；检测波长325 nm（新绿原酸、绿原酸、4,5-O-二咖啡酰奎宁酸）、334 nm（秦皮甲素、秦皮乙素、秦皮苷、秦皮素、蒙花苷）；体积流量1 mL/min；柱温30 ℃；进样量10 μL。结果 新绿原酸、秦皮甲素、绿原酸、秦皮乙素、秦皮苷、秦皮素、4,5-O-二咖啡酰奎宁酸、蒙花苷的线性范围分别为1.86～59.45、26.21～838.80、8.01～256.47、3.60～115.45、17.79～569.18、1.93～61.76、3.67～117.51、2.27～72.76 μg/mL，线性关系良好（r＝1.000 0），平均回收率分别为95.56%、92.88%、95.49%、92.15%、93.03%、96.03%、99.90%、95.42%，RSD值分别为3.40%、3.11%、3.25%、3.17%、3.10%、3.52%、3.40%、3.34%。结论 建立的芪桂痛风片中8种成分HPLC定量方法简便、快速、准确，可以用于评价芪桂痛风片的质量。

Objective To establish an HPLC method for determination of neochlorogenic acid, esculin, chlorogenic acid, esculetin, fraxin, fraxetin, 4,5-O-dicaffeoylquinic acid, and linarin in Qigui Tongfeng Tablets. Methods The separation was carried out on Ace Excel C₁₈-AR column (150 mm × 4.6 mm，3 μm). The mobile phase was acetonitrile -0.1% formic acid with gradient elution. The detection wavelength was set at 325 nm (neochlorogenic acid, chlorogenic acid, and 4,5-O-dicaffeoylquinic acid) and 334 nm (esculin, esculetin, fraxin, fraxetin, and linarin). The flow rate was 1.0 mL/min, temperature of column was set at 30 ℃, and the volume of injection was 10 μL. Results Neochlorogenic acid, esculin, chlorogenic acid, esculetin, fraxin, fraxetin, 4,5-O-dicaffeoylquinic acid, and linarin had good linear relationships in concentration range of 1.86 — 59.45, 26.21 — 838.80, 8.01 — 256.47, 3.60 — 115.45, 17.79 — 569.18, 1.93 — 61.76, 3.67 — 117.51, and 2.27 — 72.76 μg/mL (r＝1.000 0), and their average recoveries were 95.56%, 92.88%, 95.49%, 92.15%, 93.03%, 96.03%, 99.90%, and 95.42% with RSD values as 3.40%, 3.11%, 3.25%, 3.17%, 3.10%, 3.52%, 3.40%, and 3.34%, respectively. Conclusion The established HPLC quantitative method for eight components in Qigui Tongfeng Tablets is simple, rapid, and accurate, and can be used to evaluate the quality of Qigui Tongfeng Tablets.

R286.02

国家中医药管理局岐黄学者项目（2021）