目的 建立了HPLC-DAD波长切换法测定通络开痹片中5-O-甲基维斯阿米醇苷、士的宁、马钱子碱和杯苋甾酮的分析方法。方法 Purospher ODS C₁₈色谱柱（250mm×4.6mm，5μm）；以乙腈–0.5%磷酸溶液为流动相，梯度洗脱；测定波长：260nm（士的宁和马钱子碱）、243nm（杯苋甾酮）、254nm（5-O-甲基维斯阿米醇苷）；体积流量：1.0mL/min；柱温：30℃；进样体积：10μL。结果 杯苋甾酮、马钱子碱、士的宁和5-O-甲基维斯阿米醇苷分别在0.0584～5.8400、0.1180～11.8000、0.0949～9.9000、0.0238～2.3800μg/mL具有良好的线性，平均回收率分别为100.51%、99.59%、98.75%、100.38%，RSD值分别为1.6%、1.1%、1.7%、1.6%。结论 本法具有前处理简单、分析时间短、检测结果准确等优点，适用于通络开痹片的质量控制。

Objective To establish an HPLC-DAD wavelength switching method for determination of 5-O-methylvisamitol glycoside, strychnine, brucine, and cyasterone in Tongluo Kaibi Tablets. Methods Purospher ODS C₁₈ column (250 mm×4.6 mm, 5 μm) was used. The mobile phase was consisted of acetonitrile-0.5% phosphoric acid solution with gradient elution. The detection wavelengths were set at 260 nm for strychnine and brucine, 243 nm for cyasterone, and 254 nm for 5-O-methylvisamitol glycoside. The column temperature was 30℃, volume flow was 1.0 mL/min, and the injection volume was 10 μL. Results Cyasterone, brucine, strychnine, and 5-O-methylvisamitol glycoside showed good linear relationships in concentration range of 0.0584-5.8400, 0.1180-11.8000, 0.0949-9.9000, and 0.0238-2.3800 μg/mL. The average recoveries were 100.51%, 99.59%, 98.75%, and 100.38% with RSD values of 1.6%, 1.1%, 1.7%, and 1.6%, respectively. Conclusion The method has the advantages of simple pretreatment, short analysis time, and accurate detection result, which is suitable for quality control of Tongluo Kaibi Tablets.

R286.02