目的 探讨以多酚类天然产物为组方的传世逆甲片对肿瘤早筛基因甲基化的干预调控作用特点。方法 采用甲基化特异性定量聚合酶链式反应（qMSP），针对从文献中筛选得到的肿瘤早筛基因波形蛋白（VIM）、前列腺素E受体4（PTGER4）、矮小同源盒基因（SHOX2）、骨形态发生蛋白3（BMP3）、胞裂蛋白9（SEPT9）基因，设计甲基化特异性引物。选取肺癌NCI-H446细胞作为研究对象，分别经20μg/mL的姜黄素、茶多酚、茶色素、传世逆甲片和2μmol/L 5-氮杂胞苷作用后，提取全基因组DNA，得到其基因的启动子区的甲基化相对值。结果 多酚类天然产物姜黄素和5-氮杂胞苷对PTGER4、SHOX2、SEPT9的启动子甲基化具有抑制作用，而茶色素对PTGER4、BMP3、SHOX2、SEPT9基因启动子的甲基化具有促进作用，传世逆甲片对SHOX2和SEPT9甲基化抑制作用最强。结论 传世逆甲片及其含有的多酚类物质能够抑制肿瘤早期筛查基因甲基化水平，干预调控肿瘤早筛基因甲基化作用。

Objective To investigate the inhibitory effect of Nijia (TRANS-ME) Recipe, which is grouped with polyphenolic natural products, on methylation of early tumor screening genes, and to provide an experimental basis for the study of natural polyphenols in tumor prevention. Methods Quantitative methylation-specific polymerase chain reaction (qMSP) was used. Methylation-specific primers were designed for the early tumor screening genes VIM, PTGER4, SHOX2, BMP3, and SEPT9 genes obtained from literature screening. Lung cancer NCI-H446 cells were selected for the study, after being treated with 20 μg/mL curcumin, tea polyphenol, tea pigment, Nijia (TRANS-ME) Recipe, and 2 μmol/L 5-azocytoside, whole genomic DNA was extracted, and then the relative methylation values of the promoter regions of their genes were obtained. Results Curcumin and 5-azocytoside inhibit the promoter methylation of PTGER4, SHOX2 and SEPT9, while tea pigment promotes the promoter methylation of PTGER4, BMP3, SHOX2, and SEPT9. The methylation inhibition of Nijia (TRANS-ME) Recipe of SHOX2 and SEPT9 was strongest. Conclusion Nijia (TRANS-ME) Recipe and the polyphenols contained in them can inhibit the methylation level of tumor early screening genes and regulate the methylation of tumor early screening genes.

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