目的 建立HPLC-DAD法同时测定益脑胶囊中3，6'-二芥子酰基蔗糖、五味子醇甲和人参皂苷Rb₁的方法。方法 采用Agilent Eclipe Zorbax XDB C₁₈柱（250 mm×4.6 mm，5 μm）；流动相：0.1%磷酸溶液–乙腈，梯度洗脱；检测器：DAD检测器；检测波长：203 nm（人参皂苷Rb₁）、249 nm（五味子醇甲）、321 nm（3，6'-二芥子酰基蔗糖）；体积流量：1.0 mL/min；柱温：室温；进样量：10 μL。结果 3，6'-二芥子酰基蔗糖、五味子醇甲和人参皂苷Rb₁分别在0.193～19.300、0.299～29.910、0.189～188.600 μg/mL线性关系良好，平均回收率分别为99.5%、99.7%、98.3%，RSD值分别为1.2%、1.1%、0.7%。结论 本法简便、准确、快速，适用于益脑胶囊中3，6'-二芥子酰基蔗糖、五味子醇甲和人参皂苷Rb₁的测定，为完善该产品的质量标准完善提供参考。

Objective To establish an HPLC method to simultaneously determine the contents of 3,6'-dimustard acyl sucrose, schisandrin A, and ginsenoside Rb₁ in Yinao Capsules. Methods The separation was carried out on Agilent Eclipe Zorbax XDB C₁₈ column (250 mm×4.6 mm, 5 μm). Detector was ued as DAD detector. The mobile phase was 0.1% phosphoric acid solution-acetonitrile with gradient elution. The detection wavelength was set at 321 nm (3,6'-dimustard acyl sucrose), 249 nm (schisandrin A), and 203 nm (ginsenoside Rb₁). The volume flow was 1.0 mL/min, column temperature was room temperature, and injection volume was 10 μL. Results There had good linear relationships for 3,6'-dimustard acyl sucrose, schisandrin A, and ginsenoside Rb₁ within the ranges of 0.193-19.300, 0.299-29.910, and 0.189-188.600 μg/mL, whose average recoveries were 99.5%, 99.7%, and 98.3% with RSD values of 1.2%, 1.1%, and 0.7%, respectively. Conclusion The method is simple, accurate, and rapid, which is suitable for the quality control for Yinao Capsules, and provides reference for improvement of quality control of the product.

R286.02