目的 基于网络药理学方法探讨生姜治疗类风湿关节炎（RA）的可能分子作用机制。方法 通过TCMSP平台筛选生姜主要活性成分，利用UniProt数据库将相应靶点蛋白名称进行转化为基因名，经Cytoscape 3.8.2软件构建网络图；在GeneCards数据库、OMIM数据库、TTD数据库中检索“rheumatoid arthritis”，获取RA疾病靶点，制作韦恩图获取生姜药物成分和RA共同靶点，并进行蛋白质相互作用（PPI）网络分析、基因本体论（GO）功能富集及京都基因与基因组百科全书（KEGG）通路分析。利用分子对接技术预测有效成分与靶点的潜在结合活性。结果 获取生姜5个成分及55个潜在靶点，RA疾病4 836个相关靶点，生姜与RA共同靶点26个，PPI网络中自由度靠前的3个靶点基因为前列素内环氧化物合成酶2（PTGS2）、转录因子AP-1（JUN）、半胱氨酸天冬氨酸蛋白酶-3（CASP3），GO功能富集分析和KEGG通路分析分别得到185个结果和59条信号通路。结论 生姜治疗RA具有多成分、多靶点效应，并涉及多条信号通路，这可能通过PTGS2、JUN、CASP3等靶点调控炎症反应、免疫调节作用治疗RA。

Objective To investigate the possible mechanism of Zingiberis Rhizoma Recens in treatment of rheumatoid arthritis(RA)by network pharmacology. Methods The activeingredients of Zingiberis Rhizoma Recens were screened by TCMSP platform, UniProt database was used to convert the corresponding target protein name into gene name, and the network map was constructed by Cytoscape 3.8.2 software. "rheumatoid arthritis" was searched in GeneCards, OMIM, and TTD databases to obtain RA disease targets. Venn diagram was made to obtain the common targets of Zingiberis Rhizoma Recens drug components and RA. PPI network analysis, GO enrichment and KEGG pathway enrichment analysis were performed. The potential binding activity of active ingredient and target was predicted by molecular docking technique. Results Five components and 55 potential targets of Zingiberis Rhizoma Recens were obtained, including 4 836 related targets of RA disease and 26 common targets of Zingiberis Rhizoma Recens and RA. The three target genes with higher degree of freedom in PPI network were prost endocyclic oxide synthase 2 (PTGS2), transcription factor AP-1 (JUN) and cysteine aspartate protease-3 (CASP3). GO functional enrichment analysis and KEGG pathway analysis yielded 185 results and 59 signaling pathways, respectively. Conclusion Zingiberis Rhizoma Recens treatment of RA has multi-component, multi-target effect, involving multiple signaling pathways, and may regulate inflammatory response and immunomodulation through PTGS2, JUN, CASP3 and other targets to treat RA.

R285

国家自然科学基金资助项目（82160869）；贵州省科技计划项目（黔科合支撑[2021]一般006）；贵州省科技计划项目（黔科合平台人才[2020]2202号）；贵州省中医药管理局科学技术研究课题（QZYY-2020-021）；贵州省高层次创新型人才项目[（2016）5650]