目的 采用超高效液相色谱-四级杆-静电场轨道阱联用技术（UPLC-Q-Exactive-Orbitrap-MS）辨别分析白花蛇舌草注射液中化学成分。方法 采用Waters Acquity UPLC HSS T3 C₁₈色谱柱（100 mm×2.1 mm，1.8 μm），以0.1%甲酸水溶液-乙腈为流动相，梯度洗脱，柱温30℃，体积流量0.30 mL/min，样品室温度10℃，进样体积10 μL。采用电喷雾离子源（ESI），正、负离子模式下采集数据。通过NIST质谱数据库检索，结合保留时间、分子离子、主要碎片，并根据文献资料，推测其化学成分。结果 白花蛇舌草注射液中共鉴定出22个化合物，其中包括黄酮类7个、有机酸类7个，糖类3个、萜类2个、嘌呤2个、丁香脂素和酚苷各1个。结论 建立的方法能快速、准确、高效鉴定白花蛇舌草注射液中多种化学成分，为阐明白花蛇舌草的药效物质基础提供参考。

Objective To analyze chemical constituents in Baihuasheshecao Injection by UPLC-Q-Exactive-Orbitrap-MS. Methods The separation was performed on Waters Acquity UPLC HSS T3 C₁₈ column (100 mm×2.1 mm, 1.8 μm). The mobile phase was 0.1% formic acid water-acetonitrile for gradient elution. The column temperature was 30℃, flow rate was 0.30 mL/min, the sample room temperature was 10℃, and the injection volume was 10 μL. Electron spray ionization (ESI) source was adopted with both positive and negative ion modes. The chemical constituents were analyzed based on NIST mass spectrometry library, the retention time, and fragment ion peak of the compounds, as well as comparison with literature data. Results 22 Chemical constituents in Baihuasheshecao Injection were identified, including 7 flavonoids, 7 organic acids, 3 sugars, 2 terpenoids, 2 purines, 1 eugenin, and 1 phenolic glycoside. Conclusion The established method can quickly, accurately, and efficiently identify various components in Baihuasheshecao Injection, and provided a reference for elucidating the pharmacodynamic material basis of Hedyotis diffusa.

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广西卫健委自筹经费科研课题（Z20181009）；广西中药局自筹经费科研课题（GZZC2019034）