目的 探究附子泻心汤治疗慢性萎缩性胃炎的作用机制。方法 利用TCMSP数据库筛选附子泻心汤4味中药的活性成分及相应靶点；利用DisGeNet数据库筛选慢性萎缩性胃炎相关靶点，运用Cytoscape软件构建疾病–药物–靶点网络；通过STRING数据库进行蛋白互作网络（PPI）分析，得到核心靶点。利用R语言“clusterprofiler”包进行基因功能GO分析和KEGG通路富集分析。结果 共得到附子泻心汤87个活性成分，216个作用靶点，得到慢性萎缩性胃炎相关靶点639个，药物与疾病交集靶点85个，其中核心靶点包括AKT1、TP53、VEGF、TNF、PTGS2、IL6、JUN、CASP3等。附子泻心汤主要参与脂多糖应答、对细菌起源的分子应答、活性氧化物代谢、氧化应激、金属离子应答、信号通路调节等生物过程，通过调节PI3K-Akt信号通路、AGE-RAGE信号通路、乙型肝炎等通路发挥治疗慢性萎缩性胃炎的作用。结论 附子泻心汤可通过多靶点、多机制、多途径治疗慢性萎缩性胃炎。

Objective To explore the mechanism of Fuzi Xiexin Decoction in treating chronic atrophic gastritis. Methods The active components and target sites of 4 herbs of Fuzi Xiexin Decoction were screened by TCMSP database, the target sites of chronic atrophic gastritis were screened by DisGeNet database, and the disease-drug-target network was constructed by Cytoscape software PPI analysis was carried out by STRING database to get the core target, and GO analysis and KEGG pathway enrichment analysis were carried out by "clusterprofiler" package. Results There were 87 active components, 216 active targets, 639 chronic atrophic gastritis related targets, and 85 drug-disease intersection targets, the key targets included AKT1, TP53, VEGF, TNF, PTGS2, IL-6, Jun, and CASP3. Fuzi Xiexin Decoction is mainly involved in the biological processes of lipopolysaccharide response, molecular response to bacterial origin, active oxide metabolism, oxidative stress, metal ion response, signal pathway regulation, etc. PI3K-Akt signaling pathway, AGE-RAGE signaling pathway, hepatitis B pathway play a role in treatment of chronic atrophic gastritis. Conclusion Fuzi Xiexin Decoction can treat chronic atrophic gastritis through multiple targets, multiple mechanisms and multiple pathways.

R285

江苏省第五期"333工程"培养资金资助项目[苏中医科教（2018）4号]