目的 探究柚皮素对顺铂耐药宫颈癌细胞（HeLa/DDP）顺铂耐药性的影响，并探究其分子机制。方法 体外培养人HeLa/DDP细胞、HeLa细胞，CCK-8法检测不同质量浓度顺铂对HeLa/DDP、HeLa细胞增殖的影响及不同质量浓度柚皮素对HeLa/DDP细胞增殖的影响。设置对照组、顺铂组（2 mg/L顺铂）、顺铂＋柚皮素组（2 mg/L顺铂＋60 mg/L柚皮素）和顺铂＋柚皮素＋AMPK通路抑制剂组（2 mg/L顺铂＋60 mg/L柚皮素＋50 μmol/L Compound C）；CCK-8法检测各组HeLa/DDP细胞增殖情况；流式细胞仪检测各组HeLa/DDP细胞凋亡情况；蛋白免疫印迹（Western blotting）法检测各组HeLa/DDP细胞中腺苷酸活化蛋白激酶（AMPK）、p-AMPK、Bcl-2相关X蛋白（Bax）、Beclin1、微管相关蛋白1轻链3（LC3）Ⅰ、LC3Ⅱ蛋白表达情况。结果 顺铂对HeLa/DDP细胞、HeLa细胞的半数抑制浓度（IC₅₀）分别在4～8、1～2 mg/L，柚皮素对HeLa/DDP细胞的IC₅₀在30～60 mg/L。与对照组相比，顺铂组HeLa/DDP细胞增殖抑制率、凋亡率及细胞中p-AMPK/AMPK、Bax、Beclin1、LC3Ⅱ蛋白表达水平显著升高（P<0.05），LC3Ⅰ蛋白表达水平显著降低（P<0.05）；与顺铂组相比，顺铂＋柚皮素组HeLa/DDP细胞增殖抑制率、凋亡率及细胞中p-AMPK/AMPK、Bax、Beclin1、LC3Ⅱ蛋白表达水平显著升高（P<0.05），LC3Ⅰ蛋白表达水平显著降低（P<0.05）；与柚皮素组相比，AMPK通路抑制剂组HeLa/DDP细胞增殖抑制率、凋亡率及细胞中p-AMPK/AMPK、Bax、Beclin1、LC3Ⅱ蛋白表达水平显著降低（P<0.05），LC3Ⅰ蛋白表达水平显著升高（P<0.05）。结论 柚皮素可能通过激活AMPK通路介导的自噬反应，与顺铂联用发挥对HeLa/DDP细胞增殖抑制作用及凋亡促进作用。

Objective To investigate the effect of naringenin on cisplatin resistance of cervical cancer HeLa/DDP cells and its molecular mechanism.Methods HeLa/DDP cells and HeLa cells were culturedin vitro, CCK-8 method was used to detect the effects of different concentrations of cisplatin on the proliferation of HeLa/DDP and HeLa cells, and the effects of naringenin on the proliferation of HeLa/DDP cells. HeLa/DDP cells were divided into control group, cisplatin group (2 mg/L cisplatin), cisplatin + naringenin group (2 mg/L cisplatin + 60 mg/L naringenin) and cisplatin + naringenin + AMPK pathway inhibitor group (2 mg/L cisplatin + 60 mg/L naringenin + 50 μmol/L Compound C). The proliferation of HeLa/DDP cells was detected by CCK-8 method, the apoptosis of HeLa/DDP cells was detected by flow cytometry. Western blotting (WB) method was used to detect the expression of adenosine monophosphate activated protein kinase (AMPK), p-AMPK, Bcl-2 associated X protein (Bax), Beclin1, microtubule-associated protein 1 light chain 3 (LC3) I and LC3 II protein in HeLa/DDP cells.Results The IC₅₀ value of cisplatin to HeLa/DDP cells and HeLa cells were 4—8 mg/L and 1—2 mg/L, respectively. IC₅₀ value of naringenin to HeLa/DDP cells was in the range of 30—60 mg/L. Compared with those in the control group, the proliferation inhibition rate, apoptosis rate and the protein expression levels of p-AMPK/AMPK, Bax, Beclin1 and LC3 Ⅱ in HeLa/DDP cells were significantly higher in cisplatin group(P<0.05), and the expression level of LC3 Ⅰ protein was significantly lower (P<0.05). Compared with those in cisplatin group, the proliferation inhibition rate and apoptosis rate of HeLa/DDP cells and the expression levels of p-AMPK/AMPK, Bax, Beclin1 and LC3 Ⅱ protein in cisplatin + naringenin group were significantly higher, and the expression level of LC3 Ⅰ protein was significantly lower (P<0.05). Compared with those in cisplatin + naringenin group, the proliferation inhibition rate and apoptosis rate of HeLa/DDP cells and the protein expression levels of p-AMPK/AMPK, Bax, Beclin1 and LC3 Ⅱ were significantly lower in cisplatin + naringenin + AMPK pathway inhibitor group, the expression level of LC3Ⅰ protein was significantly higher (P<0.05).Conclusion Naringenin can inhibit proliferation and promote apoptosis of HeLa/DDP cells by activating autophagy mediated by AMPK pathway.

R965

国家自然科学基金资助项目（81741147）