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[摘要]
目的 研究山药Dioscorea opposita提取物联合树突细胞-细胞因子诱导的杀伤细胞(DC-CIK)对荷MDA-MB-231乳腺癌干细胞瘤裸鼠的治疗效果。方法 制备荷MDA-MB-231乳腺癌干细胞Balb/c裸鼠模型,随机分为4组,每组8只:对照组裸鼠尾iv生理盐水,0.2 mL/次,2次/周;DC-CIK组裸鼠在肿瘤干细胞接种4 d后尾iv 1×106个DC-CIK细胞,2次/周,给药3周;山药提取物组裸鼠ig山药提取物125 mg/kg,0.2 mL/d,给药3周;山药提取物联合DC-CIK组裸鼠ig山药提取物125 mg/kg,0.2 mL/d,同时尾iv 1×106个DC-CIK细胞,2次/周,给药3周。各组裸鼠在治疗3周期间每2天测量瘤体大小及裸鼠体质量,治疗结束后处死裸鼠,取出瘤体称质量;qRT-PCR法检测瘤组织中Akt信号通路中关键原癌基因c-Myc表达水平。结果 治疗结束后,各组裸鼠瘤体生长速率为山药提取物联合DC-CIK组结论 对荷MDA-MB-231乳腺癌干细胞瘤裸鼠治疗效果中,各给药组裸鼠肿瘤生长均受到明显抑制,其中以山药提取物联合DC-CIK组效果最佳。
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[Abstract]
Objective To study the therapeutic effect of Dioscoreae Rhizoma extract combined with dendritic cell-cytokine-induced killer cells (DC-CIK) on nude mice bearing MDA-MB-231 breast cancer stem cell tumor. Methods A Balb/c nude mouse model bearing MDA-MB-231 breast cancer stem cells were prepared and randomly divided into 4 groups, 8 in each group: in control group, nude mouse were administrated with normal saline by tail iv, 0.2 mL/time, 2 times/week; Nude mice in DC-CIK group were administrated with 1×106 DC-CIK cells by tail iv after 4 days of tumor stem cell inoculation, twice a week, for 3 weeks; nude mice in Dioscoreae Rhizoma extract group were administrated with Dioscoreae Rhizoma extract 125 mg/kg by ig, 0.2 mL/d, for 3 weeks; nude mice ig Dioscoreae Rhizoma extract + DC-CIK group were administrated with Dioscoreae Rhizoma extract 125 mg/kg by ig, simultaneously were administrated with 1×106 DC-CIK cells by tail iv, twice a week, administration 3 weeks. The tumor volumn and weight of nude mice in each group were measured every 2 days during treatment. After the treatment, the nude mice were sacrificed, and the tumors were taken out and weighed; qRT-PCR method was used to detect expression level of gene c-Myc, the key protocarcinoma in the Akt signaling pathway in tumor tissues. Results After the treatment, the tumor growth rate of nude mice in each group were as follows: Dioscoreae Rhizoma extract + DC-CIK group < DC-CIK group and Dioscoreae Rhizoma extract group < control group. The nude mice in Dioscoreae Rhizoma extract + DC-CIK group, DC-CIK group and Dioscoreae Rhizoma extract + DC-CIK group always had stable mood, normal eating and drinking water, and free movement; nude mice in control group were lethargic, weakened in activity, and eating and drinking decreased. At 3 weeks, the activity was blocked and the tumor was ruptured. Compared with the control group, the c-Myc gene in the tumor of nude mice in each administration group was down-regulated. Conclusion In the treatment effect of nude mice bearing MDA-MB-231 breast cancer stem cell tumor, the tumor growth of nude mice in each administration group was significantly inhibited, and the Dioscoreae Rhizoma extract + DC-CIK group had the best effect.
[中图分类号]
R285.5
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