目的 利用超高压色谱-四极杆-飞行时间串联质谱技术（UPLC-Q-TOF/MS）对大鼠ig北青龙衣乙醇提取物后在脑组织中成分分布进行分析。方法 将北青龙衣醇提物按20 mL/kg ig大鼠后，采集脑组织，采用UPLC-Q-TOF/MS法分析；正负离子模式使用ESI源对数据进行采集，并通过数据分析软件进行处理，对入脑成分分子式的鉴别采用精确质量数、同位素峰度比比较进行，进一步将其二级质谱图与对照品或数据库的二级谱图进行比对、结合已知的文献报道和对化合物的裂解规律分析，确定入脑成分的结构式。结果 从大鼠脑组织中鉴定了11种来源于北青龙衣的成分，包含7个原型成分、4个代谢产物，其中7个萘醌，2个二芳基庚烷，2个酚酸。结论 北青龙衣乙醇提取物在大鼠脑组织中分布的成分以萘醌类为主，为北青龙衣脑毒性的深入研究提供物质基础，也为其体内成分、组织分布研究提供方法学借鉴。

Objective To research the components distribution in cerebrum tissue of ethanol extract of Jugland mandshurica Maxim based on UPLC-Q-TOF/MS technology. Methods After ig 20 mL/kg of alcohol extract from extract of J. mandshurica Maxim to rats, and the tissue samples of cerebrum collected from each rat. Using the UPLC-Q-TOF/MS to detect the substance, and the obtained data was processed by data analysis software. According to the accurate mass number and isotope abundance ratio of ion fragments, the secondary mass spectrometry comparison of the database and the analysis of cracking law, the chemical molecular formula of common ions was further confirmed. Results Eleven components were obtained, including 7 prototypic components and 4 metabolites, including 7 naphthoquinones, 2 diaryl heptanes, and 2 phenolic acids. Conclusion Naphthoquinones are the main components of extract of J. mandshurica Maxim in the brain of rats, which laid a foundation for the further study on the toxicity of the brain and the study on the distribution of components and tissues in vivo.

R969.1

国家自然科学基金资助项目（81503348）；黑龙江省自然科学基金资助项目（H2017044）