目的 观察天麻素对帕金森病小鼠纹状体多巴胺（DA）、α-突触核蛋白（α-Syn）和酪氨酸羟化酶（TH）的影响及神经保护作用。方法 50只C57BL/6雄性小鼠通过ip 1-甲基-4-苯基-1，2，3，6-四氢吡啶（MPTP）建立帕金森病模型，分为模型组，天麻素低、中、高剂量组和多巴丝肼组，每组10只，另设对照组10只。天麻素低、中、高剂量组im 0.2、0.4、0.6 g/（kg·d） 天麻素注射液，多巴丝肼组im 0.05 g/（kg·d）多巴丝肼溶液，模型组、对照组im等量无菌生理盐水，连续注射14 d。末次给药后1、7、14 d对各组小鼠进行爬杆、悬挂、旷场实验等行为学测试，14 d时处死，采用分光光度法检测各组小鼠纹状体内丙二醛（MDA）和还原型谷胱甘肽（GSH）水平，Western blotting法检测各组小鼠纹状体内α-Syn和TH表达，高效液相色谱–电化学检测法检测各组小鼠DA水平。结果 相同时间下，与对照组相比，模型组小鼠爬杆时间、纹状体内MDA、α-Syn水平显著升高（P<0.05），悬挂评分、旷场活动总距离、旷场活动平均速度、纹状体内GSH、DA、TH水平显著减少（P<0.05）；与模型组相比，天麻素0.2、0.4、0.6 g/（kg·d）组和多巴丝肼组小鼠爬杆时间、纹状体内MDA、α-Syn水平显著减少（P<0.05），悬挂分值、旷场活动总距离、旷场活动平均速度、纹状体内GSH、DA、TH水平显著增多（P<0.05），天麻素0.6 g/（kg·d）组与多巴丝肼组各指标比较差异均无统计学意义。结论 天麻素可能通过提高机体抗氧化能力，保护脑内DA能神经元，从而改善帕金森病小鼠运动障碍症状。

Objective To observe the effects of gastrodin on dopamine (DA), α-synuclein (α-Syn), and tyrosine hydroxylase (TH) in striatum of Parkinson's disease mice and its neuroprotection. Methods 50 C57BL/6 Male mice were ip with 1-methyl-4-phenyl- 1,2,3,6-tetrahydropyridine (MPTP) to establish the Parkinson's disease model, and they were divided into model group, gastrodin low, middle, high dose group, and madopar group, with 10 mice in each group, and another 10 mice in the control group. The low, middle, and high dose group were im 0.2, 0.4, and 0.6 g/(kg·d) Gastrodin Injection, respectively, and the madopar group was im 0.05 g/(kg·d) madopar solution, and the model group and the control group were im the same amount of sterile saline, continuous injected for 14 days. On the 1st, 7th, and 14th days after the last administration, the mice in each group were tested by climbing pole, hanging and open field experiments, and were killed at the 14th day, malondialdehyde (MDA) and reduced glutathione (GSH) levels in striatum were measured by spectrophotometry. Western blotting was used to detect the expressions of α-Syn and TH in striatum, and the content of DA in striatum of mice in each group was detected by high performance liquid chromatography electrochemical detection (HPLC- ECD). Results At the same time, compared with the control group, the climbing time, MDA, and α-Syn levels in striatum of the model group were significantly increased (P<0.05), but suspension score, total distance of open field activity, average speed of open field activity, levels of GSH, DA, and TH in striatum decreased significantly (P<0.05). Compared with the model group, the climbing time, MDA and α-Syn levels in striatum of the gastrodin 0.2, 0.4, and 0.6 g/(kg·d) groups and the madopar group were significantly decreased (P<0.05), but suspension score, total distance of open field activity, average speed of open field activity, levels of GSH, DA, TH in striatum increased significantly (P<0.05). There was no significant difference between the gastrodin 0.6 g/(kg·d) group and madopar group. Conclusion Gastrodin may protect the DA neurons in the brain by improving the antioxidant capacity of the body, so as to improve the motor disorders of Parkinson's disease.

R966

国家自然科学基金青年基金资助项目（81600940）