目的 采用HPLC-DAD法分析大黄甘草汤剂和煎膏剂中12种药效组分。方法 采用Hypersil Gold-C₁₈色谱柱（250 mm×4.6 mm，5μm），体积流量0.8 mL/min，进样量20μL。二蒽酮类（流动相：甲醇–0.1%磷酸，梯度洗脱，时间25 min，柱温30℃，检测波长340 nm），蒽醌类（流动相：乙腈–0.1%磷酸，时间40 min，柱温20℃，检测波长254 nm），黄酮类和三萜类（流动相：乙腈–0.2%甲酸，梯度洗脱，时间55 min，柱温25℃，检测波长254、276、370 nm）。结果 2种二蒽酮类（番泻苷A、番泻苷B）和5种蒽醌类（大黄酸、大黄素、大黄酚、大黄素甲醚和芦荟大黄素）的含量在汤剂中明显低于煎膏剂。而三萜类（甘草酸）和3种黄酮类（甘草苷和异甘草苷、甘草素）的含量在汤剂中高于煎膏剂，特别是甘草苷。煎膏剂中12种成分的总量与汤剂相当。结论 煎膏剂的制备方法可能更利于大黄中二蒽酮类和蒽醌类成分的溶出或延缓了二蒽酮类成分的分解，同时抑制了甘草中三萜类和黄酮类成分的溶出或加快了这些成分的分解速度。由于二蒽酮类和蒽醌类是本方发挥药效作用的主要成分，且是君药大黄中主成分，因此大黄甘草煎膏剂可能比汤剂更有效。

Objective To analyze the twelve active components in Dahuang Gancao Decoction and Ointment by HPLC-DAD. Methods The determination was carried out on Hypersil Gold-C₁₈ column (250 mm×4.6 mm, 5 μm). The flow rate was 0.8 mL/min, and the injection volume was 20 μL. Dianthrones (The mobile phase consisted of methanol-0.1% phosphoric acid with gradient elution for 25 min. The column temperature was 30℃, and the detective wavelength was set at 340 nm). Anthraquinones (The mobile phase consisted of acetonitrile -0.1% phosphoric acid for 40 min. The column temperature was 20℃, and the detective wavelength was set at 254 nm). Flavonoids and triterpenoids (The mobile phase consisted of acetonitrile-0.2% formic acid with gradient elution for 55 min. The column temperature was 25℃, and the detective wavelength was set at 254, 276,370 nm). Results The contents of 2 kinds of dianthrones (sennoside A and sennoside B) and 5 kinds of anthraquinones (rhein, emodin, chrysophanol, physcione, and aloe-emodin) in decoction were significantly lower than those in ointment. The contents of triterpenoids (glycyrrhizic acid) and 3 kinds of flavonoids (liquiritin, isoliquiritin, and liquiritigenin) in decoction were higher than those in ointment, especially liquiritin. The total amount of 12 components in the ointment was equivalent to that in the decoction. Conclusion The preparation method of ointment may be more conducive to the dissolution of dianthrones and anthraquinones in Rhei Radix et Rhizoma or delay the decomposition of dianthrones, while inhibiting the dissolution of triterpenoids and flavonoids in Glycyrrhizae Radix et Rhizoma or accelerating the decomposition of these components. As dianthrones and anthraquinones are the main components of the prescription to play a medicinal effect, and are the main components of Glycyrrhizae Radix et Rhizoma, and Dahuang Gancao Ointment may be more effective than decoction.