目的 分析2016-2018年中国医科大学附属盛京医院铜绿假单胞菌的分布及耐药性，为临床合理使用抗菌药物提供参考。方法 收集中国医科大学附属盛京医院2016-2018年临床分离的1 465株铜绿假单胞菌，对其标本来源分布、科室分布和耐药性进行分析。结果 共分离出1 465株铜绿假单胞菌，痰液、尿液、引流液分别占53.9%、9.8%、9.1%；科室分布以ICU（23.3%）最多，其次为普外科（13.9%）、呼吸内科（13.4%）和新生儿呼吸内科（10.6%）。铜绿假单胞菌的耐药率2016-2018年变化不大，对头孢菌素类药物的耐药率较低，在12.75%~31.69%波动；对亚胺培南和美洛培南的耐药率分别为31.30%~33.46%、12.94%~29.11%，对阿米卡星的耐药率最低，为3.27%~4.54%。共分离出泛耐药铜绿假单胞菌37株，占2.5%。其中，2017年分离率最低，为1.39%。结论 应加强细菌耐药监测，采取有效措施控制耐药菌株传播，延缓泛耐药铜绿假单胞菌的出现和防止传播。

Objective To explore the distribution and drug resistance of Pseudomonas aeruginosa in Shengjing Hospital of China Medical University from 2016 to 2018, and to provide reference for rational use of antibiotics in clinic. Methods 1 465 Strains of P. aeruginosa were collected in Shengjing Hospital of China Medical University from 2016 to 2018, and the specimen source distribution, department distribution, and drug resistance were analyzed. Results A total of 1 465 strains of P. aeruginosa were isolated. The primary source included sputum (53.9%), urinary (9.8%, and drain liquid (9.1%). P. aeruginosa was mainly detected from ICU (23.3%), followed by Department of General Surgery (13.9%), Department of Respiratory Medicine (13.4%), and Department of Neonatal Respiratory Medicine (10.6%). The drug resistance rate of P. aeruginosa changed little from 2016 to 2018, and P. aeruginosa had a lower resistance rate against cephalosporin drugs, fluctuation within 12.75%-31.69%. The drug resistance rates of P. aeruginosa against imipenem and meropenem were 31.30%-33.46% and 12.94%-29.11%. The drug resistance rate of P. aeruginosa against amikacin was the lowest, with 3.27%-4.54%. 37 Strains of pandrug-resistant P. aeruginosa were isolated, accounting for 2.5%. In which, the separation rate of pandrug-resistant P. aeruginosa in 2017 was the lowest, with 1.39%. Conclution Bacterial drug resistance monitoring should be strengthened, and effective measures should be taken to control the spread of drug-resistant strains, to delay the generic drug resistance of P. aeruginosa and prevent the spread.