目的 探讨齐墩果酸抑制NLRP3炎症小体对蛛网膜下腔出血大鼠早期脑损伤的改善作用。方法 颈内动脉穿刺法构建蛛网膜下腔出血模型。成年雄性大鼠随机分为3组：假手术组、模型组、齐墩果酸20 mg/kg组，24 h后观察脑组织细胞凋亡情况；并利用Western blotting和ELISA方法检测NLRP3、caspase1、IL-1β和TNF-α的表达水平。结果 齐墩果酸20 mg/kg可以明显减少凋亡细胞的产生，能够降低NLRP3、caspase1、IL-1β和TNF-α的表达（P<0.01）。结论 齐墩果酸能够抑制NLRP3炎症小体的形成，从而改善蛛网膜下腔出血后的脑损伤。

Objective To investigate the improvement of oleanolic acid against brain cells in rats with subarachnoid hemorrhage by the suppression of NLRP3 inflammatory bodies. Methods The subarachnoid hemorrhage model was constructed by internal carotid artery puncture. SD rats were randomly divided into sham group, model group, and oleanolic acid 20 mg/kg group. The apoptosis of subarachnoid hemorrhage cells was observed, and NLRP3, caspase1, IL-1β, and TNF-α levels were detected by Western blotting and ELISA methods. Results Oleanolic acid 20 mg/kg could significantly decrease the production of apoptotic cells, and reduce the expression of NLRP3, caspase1, IL-1β, and TNF-α (P<0.01). Conclusion Oleanolic acid can inhibit the formation of NLRP3 inflammatory bodies, and can improve the brain damage after subarachnoid hemorrhage.

R285.5

辽宁省科技攻关计划项目（2015225022）