目的 探讨齐墩果酸对大鼠蛛网膜下腔出血后早期脑损伤的保护作用，阐释其作用机制。方法 72只大鼠随机分为假手术组、模型组和齐墩果酸10、20 mg/kg组，每组18只。采用颈内动脉穿刺法建立大鼠蛛网膜下腔出血模型，并在造模1 h后给药，24 h后进行神经功能损伤评分；检测脑水含率、脑伊文思蓝浸出；并利用Western blotting法检测脑组织VE-cadherin、p120-catenin蛋白的表达量。结果 齐墩果酸（20 mg/kg）可有效改善神经功能损伤（P<0.01）；降低脑水肿和脑血管通透性（P<0.01）；增加VE-cadherin、p120-catenin蛋白的表达（P<0.01）。结论 齐墩果酸对大鼠蛛网膜下腔出血后早期脑损伤具有显著的保护作用，改善脑水肿，其作用机制可能与增加脑血管内皮粘附连接蛋白的表达有关。

Objective To explore the effect of oleanolic acid on early brain injury after subarachnoid hemorrhage and explain its mechanism. Methods SD rats (72) were divided into 4 groups: Sham, model, and oleanolic acid groups (10 and 20 mg/kg), and each group had18 rats. The endovascular puncture model was used to induce subarachnoid hemorrhage. Rats were ig administered with the according drugs after 1 h of model established. Neurological score, brain water content and blood-brain barrier were evaluated after 24 h. The protein expression of VE-cadherin and p120-catenin were determined by Western blotting. Results Oleanolic acid (20 mg/kg) could significantly improve neurological scores (P < 0.01), reduced brain water content and blood-brain barrier permeability (P < 0.01), and increased the levels of VE-cadherin and p120-catenin (P < 0.01). Conclusion Oleanolic acid could protect against early brain injury after subarachnoid hemorrhage in rats and improve brain edema. The mechanism may be related to the increase of expression of endothelial adhesion protein.

辽宁省科学技术计划项目（2015225022）