目的 探讨侧脑室注射apelin-13对小鼠体温的作用及对脂多糖诱导小鼠发热的解热作用。方法 小鼠随机分为对照组,脂多糖50、150 ng组,apelin-13 3、10 nmol组,脂多糖150 ng＋apelin-13 3 nmol组和脂多糖150 ng＋apelin-13 10 nmol组。药物或生理盐水以10 μL/min的恒定速度侧脑室注射,注射体积为4 μL。给药后0.5、1.0、1.5、2.0、2.5、3.0、3.5、4.0 h分别监测记录各组小鼠的体温,计算各组小鼠在各时间点体温的变化。根据实验结果绘制温度变化曲线,并计算出曲线下面积(area under the curve,AUC)。结果 与对照组比较,脂多糖150 ng组温度均显著升高(P<0.05),引起发热效果明显,因此选择注射150 ng脂多糖作为模型组。侧脑室单独注射3、10 nmol apelin-13对小鼠温度变化没有明显影响。侧脑室注射3 nmol apelin-13在1.5 h降低150 ng脂多糖引起的发热(P<0.05);而10 nmol apelin-13在1.5、2.0、3.0 h均降低150 ng脂多糖引起的发热(P<0.05)。与脂多糖150 ng组比较,脂多糖150 ng＋apelin-13 10 nmol组体温AUC显著减少(P<0.05);脂多糖150 ng＋apelin-13 3 nmol组体温AUC虽然减少,但是并不具有显著性差异。结论 中枢apelin-13对小鼠的体温没有影响,但侧脑室注射10 nmol apelin-13能够明显降低脂多糖引起的小鼠体温升高,具有缓解脂多糖诱导的小鼠发热作用。

Objective To study the influence of introcerebroventricular (icv) administered with apelin-13 on body temperature of mice, and investigate antipyretic effect on lipopolysaccharide (LPS)-induced fever in mice. Methods Mice were randomly divided into control group, LPS 50 and 150 ng groups, apelin-13 3 and 10 nmol groups, LPS 150 ng + apelin-13 3 nmol group, and LPS 150 ng + apelin-13 10 nmol group. Drugs or normal saline were icv administered at a constant speed of 10 L/min, and the injection volume was 4 μL. Body temperature was monitored and recorded at the point of 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, and 4.0 h after treatment. The changes of body temperature in mice were calculated. Temperature change curve was drawn according to the experiment, and area under the curve (AUC) was also calculated. Results Compared with the control group, body temperature in LPS 150 ng group was significantly increased (P < 0.05), so it was selected as model group in the next experiment. There were no changes of body temperature in mice after icv administered with apelin-13 3 and 10 nmol. Apelin-13 could decrease 150 ng LPS-induced fever in mice with the dosage of 3 nmol during 1.5 h (P < 0.05), and also with the dosage of 10 nmol during 1.5, 2.0, and 3.0 h (P < 0.05). Compared with the LPS 150 ng group, the change of AUC of body temperature in LPS 150 ng + apelin-13 10 nmol group was significantly decreased (P < 0.05), but that in LPS 150 ng + apelin-13 3 nmol group had no significant differences. Conclusion Apelin-13 in central system has no effect on the body temperature of mice. But icv 10 nmol apelin-13 can relieve the body temperature of mice with LPS-induced fever, and has antipyretic effect on LPS-induced fever in mice.

河南大学科研启动经费项目(B2014094)