目的 利用基因芯片技术了解阿霉素肾病大鼠基因表达谱及其经阿奇霉素干预后的变化。方法 将81只大鼠随机分为对照组、模型组、阿奇霉素组,通过尾静脉注射法建立阿霉素肾病模型,检测第4、8周时大鼠血生化指标及24 h尿蛋白变化。利用Agilent公司生产的Agilent SurePrint G3 Rat Gene Expression(8×60 k)基因芯片检测第8周时各组大鼠的肾脏基因表达谱,并利用生物信息学方法对检测结果进行分析。结果 与模型组比较,阿奇霉素组24 h尿蛋白、总胆固醇、血肌酐均显著降低(P < 0.01),白蛋白、总蛋白、内生肌酐清除率均明显升高(P < 0.01)。通过芯片检测得到了各组大鼠的肾脏基因表达谱。与对照组比较,模型组共有185个基因发生差异性表达;而与模型比较,阿奇霉素组共有824个基因发生差异性表达。结论 阿霉素肾病的发生涉及众多基因的改变,阿奇霉素干预治疗可以改变其基因表达谱及在基因水平上使其肾组织异常的基因表达实现回归,对进一步探索阿奇霉素干预阿霉素肾病的作用机制提供了线索。

Objective To study the gene expression profile of adriamycin nephropathy rats and the changes after the intervention of azithromycin by gene chip technology. Methods Eighty-eight rats were randomly divided into control group, model group, and azithromycin group. The model was established by tail vein injection of adriamycin, and the changes of blood biochemical and 24 h urine protein in rats at 4 th and 8 th week were detected. Agilent SurePrint G3 Rat Gene Expression (8×60k) gene chip were used to detect the gene expression profile, and the results were analyzed by bioinformatics method. Results Eighty-eight rats were randomly divided into control group, model group, and azithromycin group. The model was established by tail vein injection of adriamycin, and the changes of blood biochemical and 24 h urine protein in rats at 4th and 8th week were detected. Agilent SurePrint G3 Rat Gene Expression (8×60 k) gene chip were used to detect the gene expression profile, and the results were analyzed by bioinformatics method. Conclusion The occurrence of adriamycin nephropathy involves the changes of many genes and the gene expression profile can be changed with the intervention of azithromycin, which can provide further evidence to explore the effect of azithromycin on adriamycin induced nephropathy.

天津市应用基础及前沿技术研究计划项目(13JCYBJC22300)