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[摘要]
目的 建立同时测定前列康复胶囊中大车前苷、朝藿定C、淫羊藿苷的HPLC法。方法 安捷伦Zorbax C18色谱柱(250 mm×4.6 mm,5 μm);以乙腈–0.1%磷酸水溶液为流动相,梯度洗脱;柱温35 ℃;检测波长为0~30 min,330 nm(测定大车前苷),30~50 min,270 nm(测定朝藿定C、淫羊藿苷);体积流量1.0 mL/min。结果 大车前苷、朝藿定C、淫羊藿苷分别在10.08~201.60 ng、18.43~368.60 ng、52.25~1 045.00 ng线性关系良好;平均回收率分别为100.6%、97.2%、97.2%,RSD值分别为1.5%、1.3%、1.2%。结论 该方法操作简单,重复性好,可有效的控制前列康复胶囊的质量。
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[Abstract]
Objective To establish a method for simultaneous determination of plantamajoside, epimedin C, and icariin in Qianlie Kangfu Capsules. Methods The determination was carried out on Agilent Zorbax C18 column (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of acetonitrile -0.1% phosphoric acid solution with gradient elution at a flow rate of 1.0 ml/min. The column temperature was set at 35 ℃ and the detection wavelengths were 330 nm in 0 — 30 min (determination of plantamajoside) and 270 nm in 30 — 50 min (determination of epimedin C and icariin). Results Plantamajoside, epimedin C, and icariin had good linearity in the ranges of 10.08 — 201.60 ng, 18.43 — 368.60 ng, and 52.25 — 1 045.00 ng, respectively. The average recoveries were 100.6%, 97.2%, and 97.2% with RSD 1.5%, 1.3%, and 1.2%, respectively. Conclusion The method is simple and repeatable, which can be applied to the quality control for Qianlie Kangfu Capsules.
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