目的 研究痰热清注射液对呼吸道合胞病毒感染小鼠肺损伤的保护作用,并探讨其作用机制。方法 Balb/c小鼠随机分为对照组、感染组、利巴韦林(100 mg/kg)组和痰热清注射液(2、4、8 mL/kg)组,每组10只。呼吸道合胞病毒滴鼻感染后,尾iv给予相应剂量的药物,给药容积为1 mL/100 g,1次/d,连续给药8 d。计算肺指数,测定肺组织悬液lgTCID₅₀,观察肺组织病理学改变,测定MDA、GSH含量和IFN-γ活性,检测T淋巴细胞转化功能和IL-2活性的变化。结果 经痰热清注射液治疗后,与感染组比较,痰热清注射液2、4、8 mL/kg组小鼠肺指数、lgTCID₅₀明显降低(P <0.05),呼吸道合胞病毒损伤小鼠肺组织病理状态得到改善。与感染组比较,痰热清注射液2、4、8 mL/kg组小鼠MDA水平、GSH水平明显降低,IFN-γ活性明显升高,SI明显升高(P <0.05)。结论 痰热清注射液对呼吸道合胞病毒感染小鼠的肺损伤具有保护作用,其机制可能与提高机体免疫应答能力有关。

Objective To study the protection of Tanreqing Injection against lung injury in mice induced by respiratory syncytial virus, and discuss its mechanism. Methods Balb/c mice were randomly divided into normal group, viral infection group, ribavirin (100 mg/kg) group, and Tanreqing Injection (2, 4, and 8 mL/kg) groups, and each group had 10 rats. After infected by respiratory syncytial virus, drugs were tail iv administered with volume of 1 mL/100 g, once daily, and all animals were treated for 8 d. Lung index and lgTCID₅₀ were detected, pathological changes of lung tissue were observed, level of MDA, GSH and activity of IFN-γ were determined, and T lymphocyte translation faction and IL-2 activity were detected. Results After treatment of Tanreqing Injection, compared with infection group, lung index and lgTCID₅₀ of Tanreqing Injection (2, 4, and 8 mL/kg) groups were obviously decreased (P < 0.05), and lung injury were improved. Compared with infection group, levels of MDA, GSH were significantly decreased, activity of IFN-γ and SI were significantly increased (P < 0.05). Conclusion Tanreqing Injection has protection against lung injury in mice induced by respiratory syncytial virus, which might be related to improvement of immune response ability.

天津市中医药管理局中医﹑中西医结合科研课题(13050)