目的 研究内皮抑素-力达霉素辅基蛋白融合蛋白(ES-LDP)对人乳腺癌、胰腺癌和食管癌组织的靶向性。方法 分别采用人体乳腺癌、胰腺癌和食管癌组织芯片,利用免疫组化的方法,研究ES-LDP与癌细胞的亲和力;并采用Image-Pro Plus6.0 软件计算吸光度值,进行统计分析。结果 与辅基蛋白比较,ES-LDP 对乳腺癌、胰腺癌和食管癌亲和力显著增高(P <0.001)。在胰腺癌组织,这种亲和力也显著高于对正常组织的亲和力(P <0.05)。结论 ES-LDP 对不同组织器官,亲和力有所不同;在进行体内抗肿瘤活性评价时要注意选择合适的模型。

Objective To study the targeting activity on human breast cancer, pancreatic cancer, and esophageal cancer of endostatin-lidamycin fusion protein (ES-LDP). Methods Multiple tissue microarrays of breast cancer, pancreatic cancer, and esophageal cancer were used. ES-LDP and its affinity was studied by immunohistochemical way. Additionally, the cases were analyzed by Image-Pro Plus 6.0 software. The absorbance was log transformed and mainly performed statistical analysis. Results ES-LDP had higher affinity than LDP to breast cancer, pancreatic cancer, and esophageal cancer (P < 0.001). Additionally, the affinity of ES-LDP to pancreatic tumor was higher than that of normal pancreatic tissue (P < 0.05). Conclusion The binding activity of ES-LDP is different to kinds of tissues. The suitable models are necessary to investigate its antitumor activity in vivo.

国家自然科学基金资助项目(81450049);山东省医药卫生科技发展计划资助项目(2014WS0486)