目的 研究新疆阿魏的提取物、不同极性部位以及倍半萜香豆素类化合物对人肺癌细胞的体外抑制作用,明确新疆阿魏树脂的活性成分.方法 应用MTT法检测新疆阿魏树脂提取物(100 mg/mL)、不同极性部位(100 mg/mL)和倍半萜香豆素化合物(100、50、10、1、0.1 μmol/L)对人肺癌细胞系A549、H292、H1792、H460作用48 h后生长的抑制作用,采用抑制率和IC₅₀为指标衡量其活性.结果 新疆阿魏树脂氯仿提取物在100 mg/mL质量浓度下对A549、H292、H1792、H460细胞系的抑制率分别为75.45%、65.39%、46.54%、73.68.低极性部位在100 mg/mL质量浓度下对4种细胞系的生长抑制率分别为88.74%、80.62%、60.11%、92.35%.从低极性部位中分离得到的主要倍半萜香豆素类化合物farnesiferol B、farnesiferone A、farnesiferol C、多花素宁,其中farnesiferol C、多花素宁的IC₅₀分别为35.49、15.44、44.25、21.63 μmol/L和23.60、17.90、38.33、12.35 μmol/L,表现出显著的生长抑制作用,且具有剂量相关性.结论 新疆阿魏树脂的低极性部位为体外抑制人肺癌细胞生长作用的活性部位,其所含有的高含量倍半萜香豆素化合物farnesiferol C为其主要活性成分.

Objective To study inhibitory action in vitro on human lung cancer cells of extract, different fractions, and sesquiterpene coumarins from Ferula sinkiangensis resin, and to clarify its effective constituent. Methods Using MTT method, inhibitory actions of chloroform extract (at concentration of 100 mg/mL), different polarity fractions (at concentration of 100 mg/mL), and sesquiterpene coumarins (at different concentrations of 100, 50, 10, 1, and 0.1 μmol/L) from F. sinkiangensis resin were tested on A549, H292, H1792, and H460 cell lines after 48 h co-incubation. The inhibition rate and IC₅₀ values were calculated to assay their effects. Results The chloroform extract from F. sinkiangensis resin exhibited significant inhibitory action at 100 mg/mL against A549, H292, H1792, and H460 human lung cancer cells with inhibition rates as 75.45%, 65.39%, 46.54%, and 73.68%, respectively. And the low polarity fraction had inhibitory action at 100 mg/mL against the four human lung cancer cells with inhibition rates as 88.74%, 80.62%, 60.11%, and 92.35%, respectively. Sesquiterpene coumarins farnesiferol B, farnesiferone A, farnesiferol C, and polyanthinin were isolated from low polarity fraction, and farnesiferol C and polyanthinin showed significant inhibitory action on growth of human lung cancer cells with dose dependent manner. The IC₅₀ values of farnesiferol C were 35.49, 15.44, 44.25, and 21.63 Conclusion Low polarity fraction is considered as the effective part of F. sinkiangensis resin for inhibitory activities against human lung cancer cell lines, and the sesquiterpene coumarin farnesiferol C isolated from it is main active constituent.

沈阳药科大学大学生创新创业计划项目