目的 探索黄柏、酒黄柏及盐黄柏对热证大鼠能量代谢的作用规律.方法 黄柏及其酒和盐炙品水煎液9.519、1.058 g/kg ig大鼠7 d,测定生制黄柏对2,4-二硝基苯酚所致热证大鼠肛温,以及血浆中三碘甲腺原氨酸(T₃)、四碘甲腺原氨酸(T₄)、促甲状腺激素(TSH)、促甲状腺激素释放激素(TRH)、肝组织中乳酸脱氢酶(LDH)、琥珀酸脱氢酶(SDH)、肝糖原、Na⁺-K⁺-ATP酶和Ca²⁺-Mg²⁺-ATP酶的含量.结果 生黄柏、盐黄柏均可以降低热证大鼠肛温,而酒黄柏的作用不明显.生黄柏、盐黄柏可以有效降低热证大鼠的血浆中甲状腺功能轴(T₃、T₄、TSH、TRH)和肝组织中LDH、SDH、Na⁺-K⁺-ATP酶、Ca²⁺-Mg²⁺-ATP酶的含量,并能增加热证大鼠肝组织中糖原含量,而酒炙后对能量代谢指标含量作用减弱.结论 生黄柏能够改善热证大鼠的能量代谢,且与甲状腺途径有关.盐炙后寒性增强,对热证大鼠的能量代谢有进一步的改善作用,而酒炙后,缓和黄柏的寒性,对能量代谢改善效果不显著.

Objective To explore the effect regularity of raw Phellodendri Cortex and its processed products on energy metabolism in fever rats. Methods After ig administration of water extracts from raw Phellodendri Cortex with doses of 9.519 and 1.058 g/kg for 7 d, rectal temperatures in fever rats caused by subcutaneous 2,4-dinitrophenol were determined. And then the activities of T₃, T₄, TSH, and TRH in plasma and LDH, SDH, Na⁺-K⁺-ATPase, and Ca²⁺-Mg²⁺-ATPase in liver tissue were measured. Results Raw Phellodendri Cortex and Phellodendri Cortex processed by salt could reduce rectal temperature of fever rats, but the effect of Phellodendri Cortex processed by yellow wine had no significant effect. Raw Phellodendri Cortex and Phellodendri Cortex processed by salt could effectively reduce the levels of T₃, T₄, TSH, and TRH in rat plasma and LDH, SDH, Na⁺-K⁺-ATPase, and Ca²⁺-Mg²⁺-ATPase in liver tissue. They could increase the content of glycogen in liver tissue. But the effects of Phellodendri Cortex processed by yellow wine on the contents of energy metabolism indexes were weaker. Conclusion Raw Phellodendri Cortex could improve the energy metabolism of fever rats related to thyroixine pathway. Processing by salt could strengthen these effects, while processing by yellow wine could alleviate the cold property of Phellodendri Cortex without obvious effect on the energy metabolism of fever rats.

国家自然科学基金资助项目(81274083)