目的 建立测定六经头痛片中胡薄荷酮的高效液相色谱法。方法 采用Agilent Zorbax SB C₁₈色谱柱（250 mm×4.6 mm，5 μm）；以乙腈–0.05%磷酸水溶液为流动相，进行梯度洗脱；检测波长256 nm；柱温30 ℃；体积流量1.0 mL/min；进样量10 μL。结果 胡薄荷酮在0.442 3～1 105.8 ng与峰面积线性良好（r=0.999 9），平均回收率为97.59%，RSD值为1.54%（n=6）。结论 建立的方法专属性强、准确、快速，可有效地控制六经头痛片的质量。

Objective To establish an HPLC method for the determination of pulegone in Liujing Toutong Tablets. Methods The separation of pulegone was carried out by using Agilent Zorbax SB C₁₈ column (250 mm × 4.6 mm, 5 μm) with the mobile phase containing acetonitrile - 0.05% phosphoric acid aqueous with gradient elution. The detective wavelength was set at 256 nm. The flow rate was 1.0 mL/min with injection volume of 10 μL. Results The linearity range of pulegone was 0.442 3 — 1 105.8 ng (r=0.999 9), and the average recovery was 97.59% with RSD value of 1.54% (n=6). Conclusion The method is simple and accurate with good recovery and repeatability, and suitable for the quality control of Liujing Toutong Tablets.