目的 优选夏黄颗粒处方药材的最佳提取工艺。方法 采用高效液相色谱法，建立夏黄颗粒的全息图谱以及5种蒽醌类成分的测定方法，并对水回流提取、水提醇沉、醇回流提取、水煎煮、醇提结合水提、水提醇沉结合醇渗漉6种提取工艺进行比较。以总蒽醌转移率、结合蒽醌质量为指标并结合全方全息谱优选出最佳提取方法。结果 夏黄颗粒的6种提取工艺中，醇回流提取总蒽醌转移率最高、结合蒽醌质量也最高，全息谱中各峰相对面积也较大。结论 醇回流提取工艺为夏黄颗粒处方药材的最佳工艺。

Objective To optimize the extraction technology for medicinal materials in Xiahuang Granule prescription. Methods HPLC method was used to establish the holographic spectrum as well as determination of five kinds of anthraquinones in Xiahuang Granule. Six extracting processes, such as water reflux extraction, water extraction and alcohol precipitation, alcohol reflux extraction, boiling water extraction, alcohol extract combined with water extraction, and alcohol precipitation combined with alcohol leakage process were compared to optimize extraction process. Results Ethanol extracting process had the highest yield of five anthraquinones and the highest weights of combined anthraquinones in six extracting process of Xiahuang Granule with the biggest relative area of the holographic spectrum peaks. Conclusion The optimal process is the ethanol reflux extracting process for medicinal materials in Xiahuang Granule prescription.

国家“十二五”科技支撑计划项目（2011BA107B01）；天津市科技计划项目（13ZCZDSY00200）