目的 建立薄层色谱法定性鉴别舒眠片中柴胡、白芍，并建立HPLC-ELSD法测定舒眠片中酸枣仁皂苷A的方法。方法 Diamonsil C18色谱柱（250 mm×4.6 mm，5 μm）；流动相：乙腈–0.1%磷酸水溶液，梯度洗脱；体积流量：1 mL/min；进样量：10 μL；漂移管温度：105 ℃；气体流量：2.8 L/min。结果 薄层色谱法鉴别出柴胡、白芍，酸枣仁皂苷A在20～120 μg/mL呈良好的线性关系（r＝0.999 8），平均回收率为99.75%，RSD值为0.34%。结论 柴胡、白芍的薄层色谱定性鉴别，操作简便，且阴性无干扰；酸枣仁皂苷A的HPLC-ELSD测定方法准确、简便、重复性好，可以作为舒眠片的质量控制方法。

Objective To establish a quantitative method for the identification of Bupleuri Radix and Paeoniae Alba Radix in Shumian Tablets by TLC, and the determination of jujuboside A in Shumian Tablets by HPLC-ELSD. Methods Diamonsil C18 column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase consisted of acetonitrile - 0.1% phosphoric acid water solution and the gradient elution was carried out. The flow rate was 1.0 mL/min with injection volume 10 μL. The temperature of column and drift tube was set at 105 ℃. The flow rate of gas maintained at 2.8 L/min. Results Bupleuri Radix and Paeoniae Alba Radix were identified by TLC. The linear ranges of jujuboside A was 20 — 120 μg/mL (r=0.999 8). The average recovery was 99.75% (RSD=0.34%). Conclusion The method for the identification of Bupleuri Radix and Paeoniae Alb Radix in Shumian Tablets by TLC is simple and negative sample has no interference. The method for the determination of jujuboside A is accurate, simple, and reproducible. The method can be used for the quality control of Shumian Tablets.