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[摘要]
目的 建立消肿接骨贴中芍药苷测定的方法。方法 采用高效液相色谱法外标法测定。采用Thermo C18色谱柱(250 mm×4.6 mm,5 μm);流动相:乙腈–0.1%磷酸(12∶88);体积流量:1.0 mL/min;检测波长:230 nm;柱温:25 ℃;进样量10 μL。结果 芍药苷进样量在0.124 8~1.248 0 μg呈良好的线性关系(r=0.999 8),平均回收率为98.94%,RSD值为1.73%(n=6)。结论 本研究方法专属性强,灵敏度高,简便、准确、重现性好,可用于消肿接骨贴中芍药苷的测定。
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[Abstract]
Objective To establish a method for the determination of paeoniflorin in Xiaozhong Jiegu Plaster. Methods The content of paeoniflorin was determined by HPLC with external standard method. Thermo C18 column (250 mm × 4.6 mm, 5 μm) was used with acetonitrile-0.1% orthophosphoric acid (12∶88) as the mobile phase at the flow rate of 1.0 mL/min. The detection wavelength was set at 230 nm and the column temperature was 25 ℃. The injection volume was 10 μL. Results The linear range of paeoniflorin was 0.124 8 — 1.248 0 μg (r=0.999 8) with an average recovery of 98.94% (RSD=1.73%, n=6). Conclusion The established method is specific, sensitive, simple, accurate, reproducible, and suitable for the quality control of paeoniflorin in Xiaozhong Jiegu Plaster.
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