目的 建立HPLC法同时测定养血生发胶囊中阿魏酸、2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷、羌活醇、异欧前胡素和大黄素5种活性成分的方法。方法 安捷伦Eclipse XDB-C18色谱柱（250 mm×4.6 mm，5 μm）；流动相为乙腈–0.1%冰醋酸，采用梯度洗脱；检测波长：310 nm；体积流量：1 mL/min；柱温：30 ℃；进样量：20 μL。结果 5种成分均达到基线分离，各成分均有较宽的线性范围和良好的线性关系，其平均回收率分别为95.68%、100.02%、100.55%、103.03%、100.07%。结论 该方法简便、灵敏、准确，在同一色谱检测条件下实现多指标成分同时测定，为养血生发胶囊的质量评价和控制提供新的方法。

Objective To establish a method for simultaneous determination of ferulic acid, 2,3,5,4′-tetrahydroxystibene-2-O-β- D-goucosid, notopterol, isoimperatorin and emodin in Yangxue Shengfa Capsules by HPLC. Methods The HPLC analysis was performed on an Agilent Eclipse XDB-C18 column (250 mm × 4.6 mm, 5 μm) with acetonitrile-0.1% acetate acid as mobile phase for gradient elution. The detection wavelength was set at 310 nm, and the column temperature was set at 30 ℃ with the injection volume of 20 μL at a flow rate of 1 mL/min. Results Five components were well separated and showed a good linearity. The average recoveries of them were 95.68%, 100.02%, 100.55%, 103.03%, and 100.07%. Conclusion The method is simple, sensitive, and accurate, which could provide a new method for the quality control of Yangxue Shengfa Capsule.