目的 观察消水肿膏对氢化可的松致肾阳虚大鼠肾功能改善的作用。方法 将大鼠随机分为4组，正常组，模型组，消水肿膏高、低剂量组。正常组大鼠im生理盐水1 mL/(kg·d)，其余大鼠均im氢化可的松25 mg/(kg·d)，给药体积1 mL/(kg·d)，连续8 d。消水肿膏高、低剂量组分别连续ig消水肿膏42、21 g生药/kg，2次/d，连续12 d，正常组与模型组大鼠ig相同体积纯净水。观察各组大鼠体质量变化；末次给药1 h后，用4.5%戊巴比妥钠麻醉大鼠，经腹主动脉取血，测定血清醛固酮、肌酐、尿素；取尿液测定尿蛋白、尿Na+、K+浓度；取肾脏、脾脏称量质量。结果 给药12 d，消水肿膏高、低剂量组大鼠体质量较模型组有增加趋势，但未见明显统计学差异（P＞0.05）。消水肿膏高剂量组大鼠肾脏与脾脏质量均明显高于模型组（P＜0.05），血清肌酐水平显著低于模型组（P＜0.05）。消水肿膏高剂量对尿蛋白、尿K+的影响与模型组比较无显著差异（P＞0.05），有增加尿Na+排泄量的趋势。结论 消水肿膏能够对抗氢化可的松所致的肾阳虚大鼠肾脏、脾脏质量减轻，降低其血清肌酐，可增加尿Na＋的排泄量，对肾阳虚大鼠的肾功能具有明显改善作用。

Objective To study the amelioration of Xiaoshuizhong Ointment (XO) on the renal function of kidney Yang deficiency rats induced by hydrocortisone. Methods Rats were randomly divided into four groups, including control, model, low-, and high-dose XO groups. The rats in the control group were im administered with physiological saline [1 mL/(kg·d)], others were im administered with hydrocortisone [25 mg/(kg·d)], and the volume of administration was 1 mL/(kg·d). The rats in all groups were consecutively adminstered for 8 d. The rats in low- and high-dose XO groups were ig administered with XO (21 and 42 g /kg), twice daily, for 12 d. The control and model groups were ig administered with pure water. The changes in the body weight of rats were observed. One hour after the last administration, the blood was obtained through abdominal aorta after the rats were anesthetized by 4.5% pentobarbital sodium, then the serum aldosterone, creatinine, and urea were evaluated, urine protein, concentration of Na+ and K+ in urinary were determinated, and the kidney and spleen of rats were weighed. Results After 12 d of administration, the body weight of rats in low- and high-dose XO groups showed a increasing trend compared with those in the model group without statistical significance (P＞0.05). The kidney and spleen weight of rats in high-dose XO group were significantly higher than those in the model group (P＜0.05). Serum creatinine of rats in high-dose XO group was significantly lower than that in the model group (P＜0.05). Urine protein and concentration of K+ in urinary had no significant difference between groups (P＞0.05), but high-dose XO could increase the excretion of urinary Na+. Conclusion XO could prevent kidney and spleen from weight losing of kidney Yang deficiency rats induced by hydrocortisone, reduce the serum creatinine, and increase Na+ excretion of urine.XO has obvious amelioration on the renal function of kidney Yang deficiency rats.

天津市卫生局中医、中西医结合科研课题（07054）