目的 对乌拉立肽粗品纯化并制备符合纯度要求的乌拉立肽纯品。方法 采用C18（H）ODS柱（30 mm×250 mm，10 μm）；流动相A为0.1%甲酸–水溶液，流动相B为100%乙腈；检测波长215 nm；体积流量30 mL/min；进样量60 mg。结果 RP-HPLC一步纯化后得到乌拉立肽质量分数达到98%。通过多次样品收集和冷冻干燥，制备了高纯度的乌拉立肽。结论 建立了乌拉立肽的纯化工艺，制备出符合要求的乌拉立肽纯品。

Objective To purify ularitide crude product and prepare qualified ularitide met to the purity requriement. Methods The samples were separated on C18 (H) ODS column (30 mm×250 mm, 10 μm) with 0.1% formic acid (A) - water (B) as mobile phase at a flow rate of 30 mL/min. The detection wavelegth was set at 215 nm with sample volume of 60 mg. Results The purity of ularitide could reach 98% after being purified once by RP-HPLC. The high purity ularitide was obtained after being lyophilized and collected for several times. Conclusion The purification technology is set up for ularitide and the prepared ularitide is qualified.