目的 观察番茄红素对四氯化碳（CCl4）引起的小鼠急性肝损伤的保护作用。方法 将小鼠分为正常组、模型组、番茄红素组和阳性药联苯双酯组。番茄红素组和联苯双酯组分别用番茄红素和联苯双酯ig进行预给药干预，正常组和模型组以溶剂0.1%羧甲基纤维素钠ig，连续给药7 d后ip CCl4致小鼠急性肝损伤。计算肝脏指数，检测血清中谷丙转氨酶（ALT）、谷草转氨酶（AST）以及肝组织中的超氧化歧化酶（SOD）、丙二醛（MDA）、乳酸脱氢酶（LDH）的水平，并观察肝组织HE染色切片的病理变化。结果 番茄红素能显著降低急性肝损伤小鼠血清ALT、AST活性；升高肝组织匀浆中SOD活力，降低MDA含量和LDH活性；病理切片表明给药组小鼠肝损伤均减轻。结论 番茄红素对CCl4致小鼠急性肝损伤具有保护作用，其机制可能与番茄红素所具有抗脂质过氧化和清除体内过多的氧自由基的作用有关。

Objective To study the protective effects of lycopene against carbon tetrachloride (CCl4)-induced acute hepatic injury in mice. Methods The mice were divided into four groups: normal, model, lycopene, and positive control (biphenyldicarboxylate) groups. The mice in lycopene and positive control groups were ig pretreated with lycopene and biphenyldicarboxylate once daily for consecutive 7 d, respectively. The mice in the normal and model groups were given 0.1% carboxymethylcellulose sodium (CMC-Na), the solvent, instead. Then, acute liver injury in mice was induced by ip injection of CCl4. After the mice were sacrificed, the hepatic indices were calculated. The activities of serum ALT and AST, hepatic SOD and LDH, and level of hepatic MDA were also determined. Histopathological changes of liver tissue slices stained by HE staining were observed under light microscope. Results The activities of ALT, AST and LDH, and level of MDA were significantly decreased by lycopene pretreatment, while activity of SOD in hepatic tissues was markedly increased. The effects of lycopene were close to those of biphenyldicarboxylate. Histopathological results also showed the obvious improvement of liver injury in lycopene pretreatment group. Conclusion Lycopene has protective effects against CCl4-induced acute hepatic injury in mice, and the mechanisms might be associated with its anti-oxidative activity.

国家自然科学基金资助项目（81173638，81102835，81001682）