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[摘要]
目的 在使用GC-MS技术的基础上采用直观推导式演进特征投影法(HELP)研究云南产黄水枝根、茎、叶3个不同部位的挥发油成分。方法 以黄水枝根部位为例详细介绍了HELP的解析过程,应用总体积积分法测定各成分的质量分数。结果 3个不同部位(根、茎、叶)提取的挥发油中分别鉴定出58、37、38个化合物,分别占挥发油总量的92.48%、99.16%、93.51%。结论 黄水枝不同部位的挥发油化学成分有明显差异;利用GC-MS分析法结合化学计量学分辨方法鉴定挥发油化学成分,比单独使用GC-MS法结果更准确、可靠。
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[Abstract]
Objective To study the chemical constituents of volatile oil from three different parts of Tiarella polyphylla D. Don. Methods The chemical constituents were studied with the help of heuristic evolving latent projection (HELP) resolutions based on GC-MS. The process of HELP was described in detail, while the volatile chemical constituents from roots of T. polyphylla D. Don. were analyzed. Relative contents of oil constituents were computed from the total volume integration. Results There were 58, 37, 38 compounds identified respectively from the volatile oil of root, stem, and leaf of T. polyphylla D. Don., which accounted for 92.48%,99.16%, and 93.51% respectively of the total amount of volatile oil.Conclusion There are significant differences in chemical constituents from different parts of T. polyphylla D. Don. It is more reliable and accurate to identify the chemical constituents of volatile oil by GC-MS combined with chemo-metric resolution method than by GC-MS only.
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