目的 建立同时测定金银花及金芪降糖片中绿原酸的反相高效液相色谱法。方法 色谱柱为Diamondsil C18（250 mm×4.6 mm，5 μm），以乙腈0.4%磷酸溶液（13∶87）为流动相，检测波长为327 nm，体积流量为1.0 mL/min。结果 在上述条件下测定金银花中绿原酸为2.49%，金芪降糖片中绿原酸为8.776 mg/片。结论 本方法操作简单、结果准确、回收率高、重现性好，可用于金银花及金芪降糖片的质量控制。

Objective To establish a reversedphase HPLC（RP-HPLC）method of determination of chlorogenic acid in Flos lonicerae and Jinqi Jiangtang Tablet. Methods To use RPHPLC for determination. The separation was carried out using the Diamosil C18 column (250 mm×4.6 mm，5 μm) with the mobile phase containing acetonitrile0.4% orthophosphoric acid (13∶87) and a detective wavelength at 327 nm. The flow rate was 1.0mL?min1. ResultsThe contents chlorogenic acid in Flos lonicerae and Jinqi Jiangtang Tablet were 2.49% and 8.776mg/t respectively. Conclusion The method is simple, accurate with a good recovery and repeatability. The method can be used to control the quality of Flos lonicerae and Jinqi Jiangtang Tablet.

天津市科技攻关项目（06YFGPSH02900）