目的 建立乳痈消痛片中熊果酸的HPLC测定方法。方法 采用高效液相色谱法，Kromasil C18色谱柱（250 mm×4.6 mm，5 μm）；流动相：甲醇–2%醋酸水溶液（90∶10）；体积流量：1.0 mL/min；柱温：35 ℃；检测波长：210 nm。结果 熊果酸在0.506～4.554 μg线性关系良好（r=1.000），平均加样回收率为99.95%，RSD值为0.98%（n=9）。结论 该方法灵敏度高、准确、快速、专属性强、重复性好，可用于乳痈消痛片中熊果酸的质量控制。

Objective To establish an HPLC method for determination of ursolic acid in Ruyong Xiaotong Tablet. Methods Ursolic acid was determined on Kromasil C18 column (250 mm × 4.6 mm, 5 μm) with methanol-2% acetic acid (90∶10) as mobile phase. The flow rate was 1.0 mL/min. The column temperature was 35 ℃, and the detection wavelength was 210 nm. Results The ursolic acid showed a good linear relationship in the range of 0.506—4.554 μg (r = 1.000). The average recovery of ursolic acid was 99.95% with RSD of 0.98% (n=9). Conclusion This method is accurate, fast, reliable, reproducible, and suitable for the determination of ursolic acid in Ruyong Xiaotong Tablet.