Objective Monitoring the unbound drug concentration in blood in combination therapy is necessary, because its concentration determines the efficacy of drug therapy. This study was designed to explore the effect of Dan Hong Injection (DHI) on the unbound warfarin using two approaches including an in vivo pharmacokinetic and in vitro fluorescence studies. Methods The effect of DHI on the pharmacokinetic properties of the unbound warfarin was investigated by a microdialysis sampling method coupled with LC-MS/MS. The effect of DHI and salvianolic acid B (SaB) on warfarin binding with bovine serum albumin (BSA) was conducted by fluorescence spectrometry. Results The AUC0-tn of warfarin with DHI group was higher than that of warfarin alone group. The result showed that DHI could increase the concentration of unbound warfarin in rat blood, which may be due to the competition between warfarin and DHI as well as some its components binding to serum albumin. The competition process was demonstrated by fluorescence study. Conclusion Combination therapy of DHI with warfarin could enhance the release profile of warfarin from serum protein.
关键词:
丹红注射液;荧光光谱研究;微透析;药代动力学;游离态华法林
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This work was supported by Program for Changjiang Scholars and Innovative Research Team in University (No. IRT_14R41), Tianjin Research Program of Application Foundation and Advanced Technology (15JCYBJC29300).
Jia-ding Guo, Ying-yue Zhang, Yan Huo, Yan-xu Chang, Er-wei Liu, Jia Hao. Monitoring unbound warfarin in drug combination therapy by pharmacokinetics and fluorospectrometry[J]. Chinese Herbal Medicines (CHM),2019,11(1):92-97