QR code labeling system for Xueteng-related herbs based on DNA barcode
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摘要:
目的：血藤类药材除了包括《药典》收载的鸡血藤、大血藤和滇鸡血藤以外，还包括其他皮色发红或鲜品被斩断时流出红色汁液的藤类药材。由于药材性状及中文名称相似，导致其在临床使用和商业流通中容易引起混淆。因此，亟需建立一种准确的药材鉴别标识系统，从而快速、有效的区分不同的血藤类药材。方法：对76份血藤类药材的ITS2序列进行了分析，并基于自行编写的代码和开源代码PHP QR code的编码方式将它们转换成二维DNA条形码图片。此外，基于获得的ITS2序列建立了血藤类药材的ITS2条形码参考数据库，用于25份市售血藤类药材的检测。结果：不同血藤类药材的ITS2序列长度为207-235 bp，GC含量为57.5%-71.0%。NJ系统进化树结果表明鸡血藤、大血藤和滇鸡血藤分别各自聚为一支，可有效区分。BLAST鉴定结果显示，其准确率为100%。生成的二维DNA条形码图片直观，准确的展示了不同血藤类药材的ITS2序列特征。25份市售药材的鉴定结果显示，其中22份与标签标识一致，3份标识为“滇鸡血藤”的药材，经鉴定为“鸡血藤”。结论：二维DNA条形码标识系统能够准确有效地区分市场上不同的血藤类药材，可应用于药材的流通监管。
Abstract:
Objective Xueteng-related herbs include Jixueteng, Daxueteng, Dianjixueteng, and other medicinal plant stems that release reddish-brown juices when cut. However, similarity in phonetic spelling and sound leads to their misuse in clinic or commercial circulation. Accurate labeling is imperative as well as effective species identification. Methods In this study, the ITS2 sequences of 76 samples of Xueteng-related herbs were obtained and analyzed to identify them. And then they were converted into QR codes using the open source PHP QR code. Also, a DNA barcode reference library was established according to these sequences and was used to authenticate the 25 samples of Xueteng-related herbs collected from the market. Results The lengths of the ITS2 sequences of different Xueteng-related herbs ranged from 207 to 235 bp and the GC contents were 57.5%?71.0%. Jixueteng, Daxueteng, and Dianjixueteng were clustered into three clades respectively in the neighbor-joining (NJ) phylogenetic tree, and the efficiency of the BLAST method was 100%. The ITS2 sequences of different Xueteng-related herbs were presented vividly and specifically in QR code. Twenty-two of all 25 commercial samples were consistent with the original labels, whereas three samples marked “Dianjixueteng” were authenticated as “Jixueteng”. Conclusion QR code labeling system based on DNA barcode is an effective labeling system of Xueteng-related herbs for their circulation regulation.
关键词:
血藤类药材; ITS2; DNA标识系统; 二维码; 鉴定
Keywords:
Project Supported:
We thank Dr. Yan Lu from Department of Pharmacognosy, Fudan University for providing some experimental samples. This research was supported by the CAMS Innovation Fund for Medical Sciences (CIFMS, 2016-I2M-3-016) and the Key Projects of the National Science and Technology Pillar Program (No. 2011BAI07B08).
Hong Zhou, Shuang-jiao Ma, Jing-yuan Song, Yu-lin Lin, Zheng-jun Wu, Zheng-zhou Han, Hui Yao. QR code labeling system for Xueteng-related herbs based on DNA barcode[J]. Chinese Herbal Medicines (CHM),2019,11(1):52-59
