Identification and quality analysis of Panax notoginseng and Panax vietnamensis var. fuscidicus through integrated DNA barcoding and HPLC
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摘要:
目的:三七(Panaxnotoginseng(Burk.)F. H. Chen)以其块根及根茎的治疗效果而闻名,越南三七(P. vietnamensis var.fuscidicus)被当地居民称为“野三七”或“越南三七”,已在市场上流通。由于其形态相似,可能会引起临床用药的安全问题,因而迫切需要一种快速而有效的方法进行鉴别。 方法:本研究采用DNA条形码技术对三七与越南三七进行鉴定,利用高效液相法评价二者不同组织三七皂苷R1和人参皂苷(即Rb1、Rg1、Re、Rd、Rc和Rb2)的差异。 结果:条形码结果表明三七与越南三七被聚成不同分支,越南三七与越南人参(Panaxvietnamensis)聚为一支,相似度为98%。三七与越南三七的化学成分组成在根中相似,而在须根、茎、叶和花中种类及含量均有差异。 结论:利用ITS2能快速、有效鉴别三七和越南三七;HPLC结果表明三七和越南三七的药理活性可能存在差异。
Abstract:
Objective Root or rhizome of Panax notoginseng (Sanqi) is known for its eutherapeutic effects. Panax vietnamensis var. fuscidicus, called Yesanqi or Yuenan sanqi by local residents, is also commercially available. They are similar in morphology, leading to serious safety problems in clinical medication. It is necessary to find the rapid and efficient methods to identify them. Methods P. notoginseng and P. vietnamensis var. fuscidicus were identified by DNA barcoding based on the ITS2 sequence. Notoginsenoside R1 and ginsenosides (Rb1, Rg1, Re, Rd, Rc, and Rb2) were analyzed in the roots, fibrils, stems, leaves, and flowers of P. notoginseng and P. vietnamensis var. fuscidicus using high-performance liquid chromatography (HPLC). Results P. notoginseng and P. vietnamensis var. fuscidicus were separated into branches of divergent clusters, and P. vietnamensis var. fuscidicus and Panax vietnamensis were clustered into a clade with 98% similarity according to DNA barcoding analysis. The chemical compositions of P. notoginseng and P. vietnamensis var. fuscidicus were similar in roots; while their compositions and contents of the notoginsenoside R1 and ginsenosides in flowers, leaves, stems, and fibrils were different. Conclusion ITS2 is a rapid and efficient method to identify P. notoginseng and P. vietnamensis var. fuscidicus. HPLC analysis indicated that pharmacological action might be different between P. notoginseng and P. vietnamensis var. fuscidicus.
关键词:
DNA条形码;高效液相色谱法;鉴定;三七
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We thank Prof. Zhongjian Chen for the identification of voucher speciments. This study was supported by the grants from the National Natural Science Foundation of China (No. 81603238), Beijing Nova Project (No. xx2018014), and the Major Science and Technology Programs in Yunnan Province (No. 2016ZF001-001).
Juan Yang, Lin-lin Dong, Guang-fei Wei, Hao-yu Hu, Guang-wei Zhu, Jie- Zhang, Shi-lin Chen. Identification and quality analysis of Panax notoginseng and Panax vietnamensis var. fuscidicus through integrated DNA barcoding and HPLC[J]. Chinese Herbal Medicines (CHM),2018,10(2):176-182