Metabolite Characterization of Penta-O-galloyl-??￠-D-glucose in Rat Biofluids by HPLC/QT of MS
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摘要:
Abstract:
Objective To understand the in vivo metabolic fate of 1,2,3,4,6-Penta-O-galloyl-?-D-glucose (PGG) naturally existed in many medicinal herbal and food plants such as Rhus chinensis Mill, Paeonia suffruticosa, Paeonia lactiflora and Mango. Methods The metabolites of PGG in rat biofluids were characterized using high performance liquid chromatography combined with quadrupole time-of-flight tandem mass spectrometry (HPLC/QTof-MS). Results Ten metabolites in urine, five metabolites in feces and two metabolites in plasma, were observed when the rats were administrated with a single intravenous injection of PGG (20 mg/kg). Conclusion PGG is firstly metabolized to gallic acid, then gallic acid undergoes sulfation, glucuronidation and methylation by rat liver. The determination of metabolites and the proposed metabolic pathway of PGG in vivo will be benefit to gain deeper insights into its pharmacological activities.
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Project Supported:
National Key Scientific Instrument and Development Project of China (2013YQ170525); the Program for Changjiang Scholars and Innovative Research Team in University (no. IRT15R55); National Natural Science Foundation of China (nos.81603259) and Natural Science Foundation of Shaanxi Province (2015JM2072).
Cui-xia Ma, Xue Zhao, Pei Wang, Pu Jia, Xin-feng Zhao, Chao-ni Xiao, Xiao-hui Zheng. Metabolite Characterization of Penta-O-galloyl-??￠-D-glucose in Rat Biofluids by HPLC/QT of MS[J]. Chinese Herbal Medicines (CHM),2018,10(1):73-79
