Ergosta-7,22-diene-2β,3α,9α-triol(EGDT) from Ganoderma lucidum inhibits nasopharyngeal carcinoma cells by blocking EGFR signaling pathway
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摘要:
Abstract:
Objective To investigate the efficacy and mechanism of EGDT against NPC cell lines. Methods MTT assay was used to assess cell proliferation inhibition of EGDT; The apoptotic induction and cell cycle arrest were detected by flow cytometry; Western blot was adopted to detect the protein levels; Quantitative Real-time PCR was used to determine the mRNA expressions; The NPC xenografts were established to evaluate the tumor growth inhibition of EGDT; Immunohistochemistry was applied to analyze the EGFR expression in the tumor tissues. Results EGDT showed proliferation inhibition on the NPC cell, induced G0/G1 phase arrest and cell apoptosis in vitro. EGDT decreased the protein and mRNA levels of EGFR and its downstream RAF/MEK/ERK and PI3K/AKTpathways in time- and dose-dependent manner. Furthermore, EGDT also showed a sound antitumor activity in NPC xenograft in vivo. Conclusion The treatment of EGDT displays EGFR and its mediated downstream signaling pathway blockade through decreasing the protein and mRNA levels, suggesting a promising strategy in treating human NPC.
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Youth Research Project of Health and Family Planning Commission of Fujian Province (2015-2-37).
