Progress on Research of Tissue Culture of Bletilla striata
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摘要:
Abstract:
Rhizoma Bletillae is in high demand as a traditional Chinese medicine, which natural resources have been severely damaged due to excessive exploitation. Because Bletilla striata seeds are small and have no en- dosperm, the seed germination rate is low in natural conditions. Traditional division propagation could not meet the demands of large-scale cultivation. But tissue culture can provide many seedlings in a short time and is more effective and convenient than other methods. Most studies on tissue culture of B. striata selected seeds as explants. This review summarized the processes of the aseptic seed germination path- way. It included such stages as seed germination, proliferation of clusters of buds, induction of rooting and transplanting of seedlings. Influential factors as well as optimum combination and concentration of the plant growth regulators of each stage were also summarized. The further research on tissue culture of B. striata was also prospected.
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Project Supported:
National Natural 233 Science Foundation of China (no. 81673552 ) and CAMS Innovation 234 Fund for Medical Sciences (CIFMS, 2016-I2M-3-016).
Xue-Min Wei a, Yang Liu a, Xiao-Yue Wang a, Zi-Tong Gao a, Shu-Ming Yao b, Jian-Ping Han a. Progress on Research of Tissue Culture of Bletilla striata[J]. Chinese Herbal Medicines (CHM),2018,10(1):23-26
