Li-lan Lu,Shuang Hou,Ting-ting Zheng,Xing-li Zhang,Jian-he Wei.[J].Chinese Herbal Medicines (CHM),2015,7():
Simultaneous Determination of Five Hydrophilic and Lipophilic Components from roots of Salvia miltiorrhiza by HPLC
  
DOI:
中文关键词:  
英文关键词:cryptotanshinone  danshensu  HPLC determination  protocatechuic aldehyde  Salvia miltiorrhiza  salvianolic acid B  tanshinone IIA
基金项目:
Author NameAffiliation
Li-lan Lu 1. Hainan Branch of Institute of Medicinal Plant Development (Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine), Chinese Academy of Medical Sciences, Wanning 571533, China 
Shuang Hou 2. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China 
Ting-ting Zheng 2. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China 
Xing-li Zhang 2. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China 
Jian-he Wei 1. Hainan Branch of Institute of Medicinal Plant Development (Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine), Chinese Academy of Medical Sciences, Wanning 571533, China; 2. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China 
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中文摘要:
      
英文摘要:
      Objective A reversed-phase HPLC method was established for the simultaneous determination of five hydrophilic and lipophilic components in the roots of Salvia miltiorrhiza. Methods Hydrophilic components including danshensu, protocatechuic aldehyde, and salvianolic acid B, and lipophilic components such as cryptotanshinone and tanshinone IIA, were successfully separated on a Waters Symmetry C18 reverse- phase column (250 mm × 4.6 mm, 5 μm), with acetonitrile-0.5% phosphoric acid (gradient elution) as mobile phase, the detection wavelength was set at 281 nm with flow rate of 1.0 mL/min, and the column temperature was maintained at 30 °C. Results The recovery of the method was in the range of 95.1%–102.5% and the precision was less than 3% for all five analytes. All the compounds showed good linearity (R2 > 0.9990) in a relatively wide concentration range. Therefore, this HPLC method demonstrated good reproducibility, stability, and accuracy in validation studies. Conclusion Simultaneous quanti?cation of the multiple components by HPLC would be a better strategy for the quality evaluation on the roots of S. miltiorrhiza.
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