Ming Chen,Wen-zhi Yang,Wan-ying Wu,De-an Guo.[J].Chinese Herbal Medicines (CHM),2015,7():
Chemical Analysis of Xueshuantong Lyophilized Powder by LC-MS Profiling
  
DOI:
中文关键词:  
英文关键词:fingerprint  LC-MS  Panax notoginseng  saponins  Xueshuantong
基金项目:
Author NameAffiliation
Ming Chen 1. Guangxi Wuzhou Pharmaceutical (Group) Co., Ltd., Wuzhou 543000, China 
Wen-zhi Yang 2. Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China 
Wan-ying Wu 2. Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China 
De-an Guo 2. Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China 
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中文摘要:
      
英文摘要:
      Objective To elucidate the chemical substance of Xueshuantong (XST) Lyophilized Powder and primarily disclose the chemical difference between XST and Panax notoginseng roots. Methods Liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC-ESI-MSn) was used to profile the saponins in XST and P. notoginseng. Structural elucidation was based on spectral analyses of negative and positive ESI-MS3 data, and the compare of the retention behaviors. Results The optimized LC-MS profiling approach enabled well resolution of major saponins. The negative mode ESI-MS3 fragmentation gave diagnostic information on the nature (neutral loss 162 Da for Glc, 146 Da for Rha, and 132 Da for a pentose) and sequence (priority: terminal > inner) of sugars and sapogenins (m/z 475 for protopanaxatriol; m/z 459 for protopanaxadiol), while the intact glycosyl portion could be characterized by characteristic Z0α+, Cnα+, and Cnβ+ (n = 2 or 3) obtained in the positive mode. Ultimately, totally 30 saponins were characterized from XST. Compared with the roots of P. notoginseng, three malonyl-ginsenosides, ginsenoside Rd, and gyponoside XVII (or its isomer) were almost undetectable, and showed potential significance for their differentiation. Conclusion The established LC-MS profiling approach is powerful for the chemical analysis of P. notoginseng and its preparations such as XST.
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